Purification and Characterization of a Porcine Liver Microsomal Triacylglycerol Hydrolase†
Identifieur interne : 000402 ( France/Analysis ); précédent : 000401; suivant : 000403Purification and Characterization of a Porcine Liver Microsomal Triacylglycerol Hydrolase†
Auteurs : Richard Lehner [France, Canada] ; Robert Verger [France]Source :
- Biochemistry [ 0006-2960 ] ; 1997.
Abstract
We have purified an enzyme from porcine liver microsomes which catalyzes hydrolysis of triacylglycerols. The enzyme was solubilized from the membranes by the zwitterionic detergent 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) and was purified to apparent homogeneity by sequential chromatography on Q-Sepharose, hydroxyapatite, Affi-Gel heparin, and Mono-Q. The purified hydrolase migrated in SDS−polyacrylamide gel electrophoresis (PAGE) as a single polypeptide band of an apparent molecular mass of 60 kDa. The enzyme hydrolyzed long-, medium-, and short-chain triacylglycerols, as well as a chromogenic lipase substrate, 1,2-O-dilauryl-rac-glycero-3-glutaric acid resorufin ester. The highest specific activity was obtained with tributyroylglycerol (240 μmol·min-1·mg-1). The reaction rate was maximal at pH 8.5. Sulfhydryl-directed reagents, such as N-ethylmaleimide (NEM), 5,5‘-dithiobis(2-nitrobenzoic acid) (DTNB), and dodecyldithio-5-(2-nitrobenzoic acid) (C12-TNB) had no effect on the hydrolase activity; however, the enzyme was sensitive to HgCl2. Serine reagents, such as diethyl-p-nitrophenyl phosphate (E600) and diisopropyl fluorophosphate (DFP), used in 100-fold molar excess completely inhibited the activity, suggesting that it is a serine esterase. These results suggest that the enzyme may participate in the intracellular neutral lipid metabolism since the enzyme is located in the endoplasmic reticulum, an organelle where de novo triacylglycerol synthesis and assembly of lipoproteins take place.
Url:
DOI: 10.1021/bi962186d
Affiliations:
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Hydrolase<ref type="bib" target="#bi962186dAF2"><hi rend="superscript">†</hi></ref></title><author><name sortKey="Lehner, Richard" sort="Lehner, Richard" uniqKey="Lehner R" first="Richard" last="Lehner">Richard Lehner</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Lipolyse Enzymatique, UPR 9025, GDR 1000, CNRS, 31, Chemin Joseph Aiguier, 13009 Marseille</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Canada</country><wicri:regionArea> Present address: Lipid and Lipoprotein ResearchGroup, Facultyof Medicine, The University of Alberta, Edmonton, Alberta</wicri:regionArea><wicri:noRegion>Alberta</wicri:noRegion></affiliation><affiliation></affiliation></author><author><name sortKey="Verger, Robert" sort="Verger, Robert" uniqKey="Verger R" first="Robert" last="Verger">Robert Verger</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Lipolyse Enzymatique, UPR 9025, GDR 1000, CNRS, 31, Chemin Joseph Aiguier, 13009 Marseille</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Biochemistry</title><title level="j" type="abbrev">Biochemistry</title><idno type="ISSN">0006-2960</idno><idno type="eISSN">1520-4995</idno><imprint><publisher>American Chemical Society</publisher><date type="e-published" when="1997-02-18">1997</date><date when="1997-02-18">1997</date><biblScope unit="vol">36</biblScope><biblScope unit="issue">7</biblScope><biblScope unit="page" from="1861">1861</biblScope><biblScope unit="page" to="1868">1868</biblScope></imprint><idno type="ISSN">0006-2960</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0006-2960</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">We have purified an enzyme from porcine liver microsomes which catalyzes hydrolysis of triacylglycerols. The enzyme was solubilized from the membranes by the zwitterionic detergent 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) and was purified to apparent homogeneity by sequential chromatography on Q-Sepharose, hydroxyapatite, Affi-Gel heparin, and Mono-Q. The purified hydrolase migrated in SDS−polyacrylamide gel electrophoresis (PAGE) as a single polypeptide band of an apparent molecular mass of 60 kDa. The enzyme hydrolyzed long-, medium-, and short-chain triacylglycerols, as well as a chromogenic lipase substrate, 1,2-O-dilauryl-rac-glycero-3-glutaric acid resorufin ester. The highest specific activity was obtained with tributyroylglycerol (240 μmol·min-1·mg-1). The reaction rate was maximal at pH 8.5. Sulfhydryl-directed reagents, such as N-ethylmaleimide (NEM), 5,5‘-dithiobis(2-nitrobenzoic acid) (DTNB), and dodecyldithio-5-(2-nitrobenzoic acid) (C12-TNB) had no effect on the hydrolase activity; however, the enzyme was sensitive to HgCl2. Serine reagents, such as diethyl-p-nitrophenyl phosphate (E600) and diisopropyl fluorophosphate (DFP), used in 100-fold molar excess completely inhibited the activity, suggesting that it is a serine esterase. These results suggest that the enzyme may participate in the intracellular neutral lipid metabolism since the enzyme is located in the endoplasmic reticulum, an organelle where de novo triacylglycerol synthesis and assembly of lipoproteins take place.</div></front></TEI><affiliations><list><country><li>Canada</li><li>France</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Marseille</li></settlement></list><tree><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Lehner, Richard" sort="Lehner, Richard" uniqKey="Lehner R" first="Richard" last="Lehner">Richard Lehner</name></region><name sortKey="Verger, Robert" sort="Verger, Robert" uniqKey="Verger R" first="Robert" last="Verger">Robert Verger</name></country><country name="Canada"><noRegion><name sortKey="Lehner, Richard" sort="Lehner, Richard" uniqKey="Lehner R" first="Richard" last="Lehner">Richard Lehner</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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